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GHK-Cu is a natural copper-binding tripeptide (Gly-His-Lys) first found in human blood
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High-performance liquid chromatography quantification of GSH/GSSG, 3-NT and 3-CT To evaluate biomarkers of oxidative stress and damage by high-performance liquid chromatography (HPLC), approximately 200 mg of tissue was minced and homogenized in 500 l of ice-cold phosphate-buffered saline
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Specifically, simulations suggest that blood Cys and GSH are reduced by 68.7% and 74.1% respectively, whereas cytosolic Cys and GSH are reduced by 81.2% and 64.6% respectively
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